Recombinant Mouse Chst12 Protein, Myc/DDK-tagged

• Cat.No.: Chst12-2159M
• Product Overview: Purified recombinant protein of mouse full-length carbohydrate sulfotransferase 12 (Chst12), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.
• Molecular Mass: 49.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Chst12 carbohydrate sulfotransferase 12 [ Mus musculus (house mouse) ]
• Official Symbol: Chst12
• Synonyms: CHST12; carbohydrate sulfotransferase 12; chondroitin 4-sulfotransferase 2; chondroitin 4-O-sulfotransferase 2; C4S-2; C4ST2; AI595374
• Gene ID: 59031
• mRNA Refseq: NM_021528
• Protein Refseq: NP_067503
• UniProt ID: Q99LL3