Recombinant Human CHST12, His-tagged

• Cat.No.: CHST12-11225H
• Product Overview: Recombinant Human CHST12 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 64-414a.a.
• Description: The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. Alternatively spliced transcript variants differing only in their 5 UTRs have been found for this gene.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: CHST12 carbohydrate (chondroitin 4) sulfotransferase 12 [ Homo sapiens ]
• Official Symbol: CHST12
• Synonyms: CHST12; carbohydrate (chondroitin 4) sulfotransferase 12; carbohydrate sulfotransferase 12; C4S 2; C4ST2; sulfotransferase Hlo; chondroitin 4-sulfotransferase 2; chondroitin 4-O-sulfotransferase 2; C4S-2; C4ST-2
• Gene ID: 55501
• mRNA Refseq: NM_001243794
• Protein Refseq: NP_001230723
• MIM: 610129
• UniProt ID: Q9NRB3
• Chromosome Location: 7p22
• Pathway: Glycosaminoglycan biosynthesis - chondroitin sulfate, organism-specific biosystem; Glycosaminoglycan biosynthesis - chondroitin sulfate, conserved biosystem; Sulfur metabolism, organism-specific biosystem; Sulfur metabolism, conserved biosystem; chondroitin sulfate biosynthesis, organism-specific biosystem; chondroitin sulfate biosynthesis, conserved biosystem; chondroitin sulfate biosynthesis (late stages), organism-specific biosystem
• Function: 3-phosphoadenosine 5-phosphosulfate binding; chondroitin 4-sulfotransferase activity; sulfotransferase activity; transferase activity