Recombinant Mouse Chemokine (C-X-C motif) Ligand 3

• Cat.No.: Cxcl3-373M
• Product Overview: Recombinant humanCxcl3 is a single, non-glycosylated polypeptide chain containing 69 aminoacids.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Cxcl3 is also known as MIP2β (macrophage inflammatoryprotein 2 beta), or DCIP1 (dendritic cell inflammatory protein1) in mouse,CINC2 (cytokineinduced neutrophil attractant 2) in rat, and GROγ (growthregulated oncogene gamma) in humans. It is an 8 kDa proinflammatory member ofthe CXC subfamily of heparinbinding chemokines, also called alpha chemokines.Mature mouse Cxcl3 shares 88% and 57% amino acid (aa)sequence identity with rat and human Cxcl3respectively.
• Form: Lyophilized from a0.2µm filtered concentrated solution in 1×PBS, pH 7.4.
• Molecular Weight: 7.7 kDa, a single,non-glycosylated polypeptide chain containing 69 amino acids.
• Purity: >97% by SDS-PAGEand HPLC analyses.
• Endotoxin Level: Less than 1EU/µg ofrMuDCIP-1/CXCL3 as determined by LAL method.
• Biological Activity: Fully biologicallyactive when compared to standard. Determined by its ability to chemoattract293 transfected CXCR2 cells using a concentration range of 10-100 ng/ml.
• Sequence: SELRCQCLNTLPRVDFETIQ SLTVTPPGPH CTQTEVIATL KDGQEVCLNP QGPRLQIIIK KILKSGKSS
• Reconstitution: We recommend thatthis vial be briefly centrifuged prior to opening to bring the contents tothe bottom. Reconstitute in sterile distilled water or aqueous buffercontaining 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutionsshould be apportioned into working aliquots and stored at ≤-20°C. Furtherdilutions should be made in appropriate buffered solutions.
• Storage: This lyophilizedpreparation is stable for several weeks at 2-8°C, but should be kept at -20°Cfor long term storage, preferably desiccated. Upon reconstitution, thepreparation is stable for up to one week at 2-8°C. For maximal stability,apportion the reconstituted preparation into working aliquots and store at-20°C to -80°C. Avoid repeated freeze/thaw cycles.
• OfficialSymbol: Cxcl3

Gene Information

• Gene Name: Cxcl3 chemokine (C-X-C motif)ligand 3 [ Mus musculus ]
• Synonyms: Cxcl3; chemokine(C-X-C motif) ligand 3; Dcip1; Gm1960; C-X-C motif chemokine 3; dendriticcell inflammatory protein 1
• Gene ID: 330122
• mRNA Refseq: NM_203320
• Protein Refseq: NP_976065
• UniProt ID: Q6W5C0
• Chromosome Location: 5 E1; 5
• Pathway: Chemokine receptorsbind chemokines; Class A/1 (Rhodopsin-like receptors); Cytokines andInflammatory Response (BioCarta); G alpha (i) signalling events; GPCRdownstream signaling; GPCR ligand binding; Peptide ligand-binding receptors;Senescence and Autophagy; Signal Transduction; Signaling by GPCR
• Function: chemokine activity;cytokine activity; protein binding