Recombinant Mouse Chek1, His & GST tagged

• Cat.No.: Chek1-8670M
• Product Overview: Recombinant Mouse Chek1 (O35280-1) (Met1-Thr476), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.

Specification

• Source: Insect cells
• Species: Mouse
• Tag: His&GST
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse CHEK1/GST chimera consists of 713 amino acids and has a calculated molecular mass of 82.2 kDa. The recombinant protein migrates as an approximately 78 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Met1-Thr476
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Chek1 checkpoint kinase 1 [ Mus musculus ]
• Official Symbol: Chek1
• Synonyms: CHEK1; checkpoint kinase 1; serine/threonine-protein kinase Chk1; rad27 homolog; checkpoint kinase-1; CHK1 checkpoint homolog; checkpoint kinase 1 homolog; Chk1; rad27; C85740
• Gene ID: 12649
• mRNA Refseq: NM_007691
• Protein Refseq: NP_031717