CHK1 Checkpoint Homolog (S. pombe)

• Cat.No.: CHEK1-1125H
• Product Overview: Recombinant human CHEK1 was expressed by baculovirus in Sf9 insect cells.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Non
• Description: CHEK1 is a 56 kd serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle. CHEK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia. In vitro, CHEK1 binds to and phosphorylates the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases.
• Biological Activity: 369 nmol phosphate incorporated into CHKtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 μCi/assay).
• Molecular Weight: 82.0 kDa
• Form: Recombinant protein in storage buffer (50 mMTris-HCl, pH 7.5, 150 mMNaCl, 0.25 mM DTT, 0.1 MmEGTA, 0.1 mMEDTA, 0.1 mM PMSF, 30% glycerol).
• Appearance: Liquid
• Purity: ≥90% by SDS-PAGE
• Storage: Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoidrepeated thawing and freezing.
• Pathways: Activation of ATR in response to replication stress; Cell Cycle Checkpoints; Cell cycle; Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex; Circadian rhythm pathway; G1/S DNA Damage Checkpoints; G2/M Checkpoints; G2/M DNA damage checkpoint; HTLV-I infection; IL-3 Signaling Pathway; Ubiquitin Mediated Degradation of Phosphorylated Cdc25A; p53 pathway; p53 signaling; p53-Independent DNA Damage Response; p53-Independent G1/S DNA damage checkpoint

Gene Information

• Gene Name: CHEK1 CHK1 checkpoint homolog (S. pombe) [ Homo sapiens ]
• Official Symbol: CHEK1
• Synonyms: CHEK1; CHK1 checkpoint homolog (S. pombe); CHK1; CHK1 (checkpoint, S.pombe) homolog; Serine/threonine-protein kinase Chk1; EC 2.7.11.1; OTTHUMP00000231300; OTTHUMP00000231301; OTTHUMP00000231306; OTTHUMP00000231307; OTTHUMP00000231309; Checkpoint, S. pombe, homolog of, 1; EC 2.7.11
• Gene ID: 1111
• mRNA Refseq: NM_001114121
• Protein Refseq: NP_001107593
• MIM: 603078
• UniProt ID: O14757
• Chromosome Location: 11q24.2
• Function: BMP receptor binding; cytokine activity; growth factor activity; receptor binding