Recombinant Human CHEK1, His-tagged

• Cat.No.: CHEK1-26714TH
• Product Overview: Recombinant full length human Chk1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag, 59kDa.

Specification

• Description: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
• Conjugation: HIS
• Biological activity: Specific activity: 144 nmol /min/mgas determined by Kinase assay.
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Full Length: Full L.

Gene Information

• Gene Name: CHEK1 checkpoint kinase 1 [ Homo sapiens ]
• Official Symbol: CHEK1
• Synonyms: CHEK1; checkpoint kinase 1; CHK1 (checkpoint, S.pombe) homolog; serine/threonine-protein kinase Chk1; CHK1
• Gene ID: 1111
• mRNA Refseq: NM_001114121
• Protein Refseq: NP_001107593
• MIM: 603078
• Uniprot ID: O14757
• Chromosome Location: 11q24.2
• Pathway: Activation of ATR in response to replication stress, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem
• Function: ATP binding; histone kinase activity (H3-T11 specific); nucleotide binding; protein binding; protein serine/threonine kinase activity