Recombinant Mouse Cdh5 protein, His & T7-tagged

• Cat.No.: Cdh5-6863M
• Product Overview: Recombinant Mouse Cdh5 aa. (Gly67~Gly390 (Accession # P55284)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. Mice lacking the encoded protein die in utero due to vascular insufficiency, caused by increased endothelial apoptosis. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 8.
• Source: E. coli
• Species: Mouse
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 40.4kDa.
• Protein length: Gly67~Gly390
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.3.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Cdh5 cadherin 5 [ Mus musculus (house mouse) ]
• Official Symbol: Cdh5
• Synonyms: 7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225; 7B4/cadherin-5; VE-cadherin; vascular endothelial cadherin; cadherin-5
• Gene ID: 12562
• mRNA Refseq: NM_009868.4
• Protein Refseq: NP_033998.2
• UniProt ID: P55284