Recombinant Human CDH5 protein, His & T7-tagged

• Cat.No.: CDH5-6862H
• Product Overview: Recombinant Human CDH5 aa. (Gly69~Gly391 (Accession # P33151)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 40.0kDa.
• Protein length: Gly69~Gly391
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 4.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: CDH5 cadherin 5 [ Homo sapiens (human) ]
• Official Symbol: CDH5
• Synonyms: 7B4; CD144; 7B4 antigen; VE-cadherin; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin-5
• Gene ID: 1003
• mRNA Refseq: NM_001795.4
• Protein Refseq: NP_001786.2
• UniProt ID: P33151