Recombinant Mouse CD3E & CD3D protein, His-Avi-tagged & Tag Free, Biotinylated

• Cat.No.: CD3E & CD3D-646MB
• Product Overview: Biotinylated Mouse CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free is expressed from human HEK293 cells. It contains AA Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P22646-1(CD3E) & P04235-1(CD3D)). It is the biotinylated form of Mouse CD3 epsilon & CD3 delta Protein.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&His
• Protein Length: Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D)
• Description: T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
• Predicted N Terminal: Asp 23 (CD3E) & Phe 22 (CD3D)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Molecular Mass: Biotinylated Mouse CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 17.3 kDa (CD3E) and 13.3 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag and followed by an Avi tag and subunit CD3D contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 20-22 kDa due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Cd3e CD3 antigen, epsilon polypeptide [ Mus musculus (house mouse) ]
• Official Symbol: CD3E; CD3D
• Synonyms: CD3E & CD3D, CD3 delta & CD3 epsilon, CD3 delta/epsilon
• Gene ID: 12501
• mRNA Refseq: NM_007648.5
• Protein Refseq: NP_031674.1
• UniProt ID: P22646