Active Recombinant Human CD3E & CD3D Protein, His-Avi-tagged, Biotinylated

• Cat.No.: CD3E & CD3D-377H
• Product Overview: Biotinylated Active Recombinant Human CD3E & CD3D Protein(Asp 23-Asp 126&Phe 22-Ala 105)(P07766-1&P04234-1), fused with a polyhistidine tag and an Avi tag at the C-terminus, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Asp 23-Asp 126&Phe 22-Ala 105
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
• Bio-activity: Immobilized OKT3 at 10 μg/mL (100 μL/well) can bind Biotinylated Human CD3E & CD3D with a linear range of 0.156-5 ug/mL.
• Molecular Mass: Protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 18.7 kDa (CD3E) and 13.5 kDa (CD3D).
• Endotoxin: <1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90% as determined by SDS-PAGE and SEC-HPLC.
• Storage: Store it under sterile conditions at -20 to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.385 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• GeneID 2: 915
• Publications:
  ISB 2001 trispecific T cell engager shows strong tumor cytotoxicity and overcomes immune escape mechanisms of multiple myeloma cells (2024)

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• UniProt ID: P07766
• Gene Name 2: CD3D CD3d molecule, delta (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol 2: CD3D
• Synonyms 2: CD3D; CD3d molecule, delta (CD3-TCR complex); CD3d antigen, delta polypeptide (TiT3 complex) , T3D; T-cell surface glycoprotein CD3 delta chain; CD3 delta; OKT3, delta chain; CD3 antigen, delta subunit; T-cell receptor T3 delta chain; CD3d antigen, delta polypeptide (TiT3 complex); T3D; CD3-DELTA
• mRNA Refseq 2: NM_000732
• Protein Refseq 2: NP_000723
• MIM 2: 186790
• UniProt ID 2: P04234