Recombinant Mouse CD300C Chimera Protein, N-Fc-tagged

• Cat.No.: CD300C-13M
• Product Overview: Recombinant Mouse CD300C Chimera Protein, fused to Fc-tag at N-terminus, was expressed in CHO.

Specification

• Description: CMRF-35-like molecule-6 (CLM-6), known as LMIR8, CMRF-35A1, and CD300c, is a type I transmembrane glycoprotein belonging to the immunoregulatory signaling (IRS) family of immunoglobulin-like molecules. There have been 7 human and 9 mouse CD300/CLM family members identified, and they modulate a broad and diverse array of immune cell processes via their paired activating and inhibitory receptor functions. Mouse CD300c consists of an extracellular domain (ECD) with one Ig-like domain, a single transmembrane segment, and a short cytoplasmic tail. The mature ECD of mouse CD300c is 167 amino acids and shares 48% sequence identity with human CD300c. A charged amino acid residue in the transmembrane domain of CD300c/CLM-6, found in additional family members CLM‑2, CLM-4, CLM-5, and CLM-7, enables interactions with adapter proteins. CD300c has been shown to be a FcR gamma -coupled receptor that is selectively expressed in Plasmacytoid dendritic cells (pDCs). CD300c signals might negatively regulate the activation of pDCs in response to viral infections. Additional research into CD300 receptor function during viral infections could help develop novel anti-viral therapies.
• Source: CHO
• Species: Mouse
• Tag: Fc
• Protein length: His22-Pro187
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.8-4.8 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 45 kDa
  SDS-PAGE: 55-70 kDa, reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: Cd300c CD300C molecule [ Mus musculus (house mouse) ]
• Official Symbol: CD300C
• Synonyms: CD300C; CD300C molecule; Clm6; CD300c antigenCMRF35 antigen; CLM6; CLM-6; CMRF35 leukocyte immunoglobulin-like receptor; CMRF35; CMRF-35; CMRF35A leukocyte immunoglobulin-like receptor; CMRF-35A; CMRF35ACMRF35A1; CMRF35CMRF35-A1; CMRF35-like molecule 6; IGSF16; IgSF16; IGSF16CD300 antigen-like family member C; Immunoglobulin superfamily member 16; LIR
• Gene ID: 387565
• mRNA Refseq: NM_001368239.1
• Protein Refseq: NP_001355168.1
• UniProt ID: A2A7V7