Recombinant Mouse Cd274 Protein, Fc/His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Cd274-593MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Cd274 extracellular domain (Met 1-Thr 238) (NP_068693.1), fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus, was produced in Human Cell.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&His
• Protein Length: 468
• Form: Lyophilized
• Molecular Mass: The recombinant mouse PD-L1/Fc is a disulfide-linked homodimeric protein after proteolytic removal of the signal peptide. The reduced monomer consists of 468 amino acids and predicts a molecular mass of 52.8 kDa. As a result of glycosylation, the apparent molecular mass of rm PD-L1/Fc monomer is approximately 65-75 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Cd274 CD274 antigen [ Mus musculus ]
• Official Symbol: Cd274
• Gene ID: 60533
• mRNA Refseq: NM_021893
• Protein Refseq: NP_068693