Active Recombinant Cynomolgus / Rhesus macaque CD274 protein, His-Avi-tagged, Biotinylated

• Cat.No.: CD274-0619C
• Product Overview: Biotinylated Recombinant Cynomolgus / Rhesus macaque CD274 protein(Phe19-Arg238), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Cynomolgus/Rhesus macaque
• Source: HEK293
• Tag: Avi&His
• Protein Length: 19-238 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Cynomolgus / Rhesus macaque PD-L1, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human PD-1, Fc Tag, low endotoxin with a linear range of 0.3-2 ng/mL. Immobilized Biotinylated Cynomolgus / Rhesus macaque PD-L1, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Cynomolgus PD-1, Fc Tag with a linear range of 0.3-5 ng/mL. Immobilized Biotinylated Cynomolgus / Rhesus macaque PD-L1, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-PD-L1 Neutralizing Antibody with a linear range of 0.1-3 ng/mL. Immobilized Anti-PD-L1 Neutralizing Antibody at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque PD-L1, His,Avitag with a linear range of 0.2-10 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 28.8 kDa. The protein migrates as 32-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Phe19-Arg238
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.