Recombinant Mouse Ccl17 protein

• Cat.No.: Ccl17-622M
• Product Overview: Recombinant Mouse Ccl17 protein was expressed in Escherichia coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Protein Length: 70
• Description: Murine CCL17 also known as TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine and SCYA17, is encoded by the CCL17 gene located on the chromosome 8. Among CC chemokine family members, CCL17 has approximately 24 – 29 % amino acid sequence identity with RANTES, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3 and I-309. CCL17 is a secreted protein and expressed by thymus cells constitutively and phytohemagglutinin-stimulated peripheral blood mononuclear cells transiently. It signals through the chemokine receptors CCR4 and CCR8 and displays chemotactic activity for T lymphocytes and some other leukocytes. CCL17 play an important role in skin diseases such as atopic dermatitis, bullous pemphigoid and mycosis fungoides.Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils.
• Form: Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
• Bio-activity: Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.
• Molecular Mass: Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
• AA Sequence: ARATNVGRECCLDYFKGAIPIRKLVSWYKTSVECSRDAIVFLTVQGKLICADPKDKHVKKAIRLVKNPRP
• Endotoxin: Less than 1 EU/µg of rMuTARC/CCL17 as determined by LAL method.
• Purity: >97% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: Ccl17
• Official Symbol: Ccl17
• Synonyms: CCL17; chemokine (C-C motif) ligand 17; small inducible cytokine subfamily A17; Tarc; Abcd-2; Scya17; Scya17l
• Gene ID: 20295
• mRNA Refseq: NM_011332
• Protein Refseq: NP_035462
• UniProt ID: F6R5P4