Recombinant Mouse Ccl12 protein

• Cat.No.: Ccl12-621M
• Product Overview: Recombinant Mouse Ccl12 protein was expressed in Escherichia coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Protein Length: 82
• Description: Murine CCL12 is belonging to the CC chemokine family. It shows 66 % amino acid sequence identity to human MCP-1. CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, it will also chemoattract eosinophils. CCL12 can induce a calcium flux in human HEK-293 cells transfected with murine CCR2, but can not induce a calcium flux in HEK-293 cells transfected with CCR1, CCR3, or CCR5. That shows the functional receptor of CCL12 is CCR2.
• Form: Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
• Bio-activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml.
• Molecular Mass: Approximately 9.3 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids.
• AA Sequence: GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG
• Endotoxin: Less than 1 EU/μg of rMuMCP-5/CCL12 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: Ccl12
• Official Symbol: Ccl12
• Synonyms: CCL12; chemokine (C-C motif) ligand 12; C-C motif chemokine 12; MCP-1-related chemokine; small inducible cytokine A12; small-inducible cytokine A12; monocyte chemotactic protein 5; monocyte chemoattractant protein 5; MCP-5; Scya12
• Gene ID: 20293
• mRNA Refseq: NM_011331
• Protein Refseq: NP_035461
• UniProt ID: Q62401