Active Recombinant Mouse Ccl12 Protein (82 aa)

• Cat.No.: Ccl12-224C
• Product Overview: Recombinant Mouse MCP-5/CCL1 produced in CHO cells is a polypeptide chain containing 82 amino acids. A fully biologically active molecule, rmMCP-5/CCL12 has a molecular mass of 11 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.

Specification

• Species: Mouse
• Source: CHO
• Protein Length: 82
• Description: Monocyte Chemotactic Protein-5(MCP-5), also known as Chemokine (C-C motif) ligand 12(CCL12), is a small cytokine belonging to the CC chemokine family that has been characterized in mice. Due to its similarity with human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. MCP-5 specifically attracts eosinophils, monocytes and lymphocytes.It can signal through the CCR2 receptor.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: The EC50 value of mouse MCP-5/CCL12 on Ca^2+ mobilization assay in CHO-K1/Gα15/mCCR2 cells (human Gα15 and mouse CCR2 stably expressed in CHO-K1 cells) is less than 0.5 μg/mL.
• Molecular Mass: 9.3-11 kDa, observed by reducing SDS-PAGE.
• AA Sequence: GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 98% as analyzed by SDS-PAGE.
• Storage: Lyophilized recombinant Mouse MCP-5/CCL12 remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, Mouse MCP-5/CCL12 should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Gene Information

• Gene Name: Ccl12 chemokine (C-C motif) ligand 12 [ Mus musculus ]
• Official Symbol: Ccl12
• Synonyms: CCL12; chemokine (C-C motif) ligand 12; C-C motif chemokine 12; MCP-1-related chemokine; small inducible cytokine A12; small-inducible cytokine A12; monocyte chemotactic protein 5; monocyte chemoattractant protein 5; MCP-5; Scya12
• Gene ID: 20293
• mRNA Refseq: NM_011331
• Protein Refseq: NP_035461
• UniProt ID: Q545B5