Recombinant Mouse Casc3 Protein, Myc/DDK-tagged

• Cat.No.: Casc3-1969M
• Product Overview: Purified recombinant protein of mouse full-length cancer susceptibility candidate 3 (Casc3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Required for pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA-helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favoring cell recovery following stress. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 75.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Casc3 cancer susceptibility candidate 3 [ Mus musculus (house mouse) ]
• Official Symbol: Casc3
• Synonyms: CASC3; cancer susceptibility candidate 3; protein CASC3; mBtz; Barentsz; MLN 51 homolog; protein barentsz; metastatic lymph node 51; metastatic lymph node gene 51 protein homolog; cancer susceptibility candidate gene 3 protein homolog; Btz; Mln51
• Gene ID: 192160
• mRNA Refseq: NM_138660
• Protein Refseq: NP_619601
• UniProt ID: Q8K3W3