Recombinant Mouse Bid Protein, MYC/DDK-tagged

• Cat.No.: Bid-711M
• Product Overview: Purified recombinant protein of full-length mouse BH3 interacting domain death agonist (Bid), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Ubiquitous expression in kidney adult (RPKM 20.6), thymus adult (RPKM 10.6) and 26 other tissues.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 22 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Bid BH3 interacting domain death agonist [ Mus musculus (house mouse) ]
• Official Symbol: Bid
• Synonyms: Bid; BH3 interacting domain death agonist; AI875481; AU022477; 2700049M22Rik; BH3-interacting domain death agonist; p22 BID
• Gene ID: 12122
• mRNA Refseq: NM_007544
• Protein Refseq: NP_031570
• UniProt ID: P70444

Reviews

12/19/2021

Activity retention time is very long and can be maintained for a long time.

09/13/2021

The detection method is very sensitive and can easily detect small amounts of protein.

04/10/2021

Expression level and stability are good and will be repurchased.

Q&As

In which cellular processes does the BID protein play?

BID protein mainly plays a role in the process of apoptosis. It can regulate cell survival and death signaling by interacting with other members of the Bcl-2 family.

Can the expression level and activity of BID protein be determined?

The expression level of BID protein can be detected by immunohistochemistry, western blotting, real-time quantitative PCR and other techniques. At the same time, the activity of BID protein can be assessed by detecting the level of phosphorylation of BID protein and the number of BID protein within cells.

How are BID proteins involved in the process of apoptosis?

During apoptosis, the BID protein is activated, forming complexes with other Bcl-2 family members, releasing cytochrome c, initiating a cascade of caspase enzymes, and ultimately leading to apoptosis.

How are the interactions between BID proteins and other proteins regulated?

The interaction between BID protein and other proteins is regulated by a variety of factors, including phosphorylation, methylation, acetylation and other modifications, as well as interactions with other proteins. These factors can affect the interaction between BID protein and other proteins, which in turn affects the regulatory process of apoptosis.

How can BID proteins be used as biomarkers in clinical practice?

In clinical practice, the expression level of BID protein can be measured to predict disease progression and prognosis, and to guide the choice of treatment regimen. For example, in some tumor diseases, measuring the expression level of BID protein can help doctors determine the type, stage, and prognosis of the disease, and guide the choice of treatment regimen.

Can the expression level of BID protein be regulated?

Expression level of BID protein is regulated by a variety of factors, including gene transcription, translation, modification, and degradation. These factors can affect the expression level of BID protein, which in turn affects the survival and death signals of cells.