Recombinant Mouse Atg7 Protein, His-tagged

• Cat.No.: Atg7-1525M
• Product Overview: Recombinant Mouse Atg7 Protein (Arg477~Val698) with N-terminal His-tag was expressed in E.coli.

Specification

• Description: This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 27.8 kDa
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles.
  Store at 2-8 centigrade for one month.
  Aliquot and store at -80 centigrade for 12 months.
• Concentration: 200 µg/mL
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex
• Protein length: 477-698 a.a.

Gene Information

• Gene Name: Atg7 autophagy related 7 [ Mus musculus (house mouse) ]
• Official Symbol: Atg7
• Synonyms: Atg7; autophagy related 7; Agp7; Apg7l; Atg7l; Gm21553; 1810013K23Rik; ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; autophagy-related protein 7; ubiquitin-activating enzyme E1-like protein
• Gene ID: 74244
• mRNA Refseq: NM_028835
• Protein Refseq: NP_083111
• UniProt ID: Q9D906