Recombinant Mouse Atg4b Protein, Myc/DDK-tagged

• Cat.No.: Atg4b-1760M
• Product Overview: Purified recombinant protein of mouse full-length autophagy related 4B, cysteine peptidase (Atg4b), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 44.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Atg4b autophagy related 4B, cysteine peptidase [ Mus musculus (house mouse) ]
• Official Symbol: Atg4b
• Synonyms: ATG4B; autophagy-related 4B (yeast); cysteine protease ATG4B; autophagin 1; autophagin-1; AUT-like 1 cysteine endopeptidase; AUT-like 1, cysteine endopeptidase; autophagy-related protein 4 homolog B; APG4 (ATG4) autophagy-related homolog B; autophagy-related cysteine endopeptidase 1; cysteine protease involved in autophagy APG4-B; Apg4b; Autl1; Atg4bl; AW048066; 2510009N07Rik
• Gene ID: 66615
• mRNA Refseq: NM_174874
• Protein Refseq: NP_777363
• UniProt ID: Q8BGE6