Recombinant Mouse APLN protein(Met1-Phe77), mFc-tagged

• Cat.No.: APLN-2368M
• Product Overview: Recombinant Mouse APLN (Q9R0R4) (Met1-Phe77) was expressed in HEK293 with the Fc region of Mouse IgG2a at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: mFc
• Protein length: Met1-Phe77
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant Mouse APLN consists of 297 amino acids and predicts a molecular mass of 33.57 kDa. It migrates as an approximately 40.07 kDa, 38.40 kDa and 31.65 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Apln apelin [ Mus musculus ]
• Official Symbol: APLN
• Synonyms: APLN; apelin; preproapelin; APJ endogenous ligand; Apel; 6030430G11Rik
• Gene ID: 30878
• mRNA Refseq: NM_013912
• Protein Refseq: NP_038940