Recombinant Mouse Ang protein, His & GST-tagged

• Cat.No.: Ang-520M
• Product Overview: Recombinant Mouse Ang (P21570) aa. (Gln25~Leu145) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: GST&His
• Protein Length: Gln25~Leu145
• Description: This gene encodes a member of the pancreatic ribonuclease A superfamily and is a potent inducer of neovascularization. The encoded protein is a secreted multifunctional tRNA-specific ribonuclease that promotes angiogenesis in response to angiogenetic stimuli such as hypoxia, mediates stress-induced translational repression by cleaving cellular tRNAs, stimulates cell proliferation by mediating rRNA transcription in prostate cancer cells, and is involved in neurite pathfinding. This gene resides in a cluster of highly related genes. It shares dual promoters and 5' exons with the ribonuclease, RNase A family 4 gene. Two alternatively spliced variants, with different 5' exons but the same coding exon, have been identified. Multiple pseudogenes have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 45.7kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 8.2.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4

Gene Information

• Gene Name: Ang angiogenin, ribonuclease, RNase A family, 5 [ Mus musculus (house mouse) ]
• Official Symbol: Ang
• Synonyms: Ang; angiogenin, ribonuclease, RNase A family, 5; Ang1; Rnase5; Rnase5a; AI385586; angiogenin, ribonuclease A family, member 1; RNase 5; angiogenin-1; ribonuclease 5
• Gene ID: 11727
• mRNA Refseq: NM_001161731.2
• Protein Refseq: NP_001155203.1
• UniProt ID: P21570