Recombinant Mouse ACVR2A protein(Met1-Pro134), His-tagged

• Cat.No.: Acvr2a-492M
• Product Overview: Recombinant Mouse ACVR2A (NP_031422.3) (Met 1-Pro 134) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-134 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.6-3 μg/mL in the presence of 10 ng/ml Recombinant Human Activin A.
• Molecular Mass: The secreted recombinant mouse ACVR2A comprises 126 amino acids and has a calculated molecular mass of 14.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Acvr2a activin receptor IIA [ Mus musculus ]
• Official Symbol: ACVR2A
• Synonyms: ACVR2A; activin receptor IIA; activin receptor type-2A; ACTR-IIA; activin receptor type IIA; Acvr2; ActrIIa; TactrII
• Gene ID: 11480
• mRNA Refseq: NM_007396
• Protein Refseq: NP_031422