Recombinant Mouse ACO2 Protein, GST & His-tagged

• Cat.No.: ACO2-950M
• Product Overview: Recombinant mouse ACO2 (Q99KI0) (Gln 28-Gln 780) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 28-780 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% gly, 0.5mM GSH, pH 8.01, 5%~8% trehalose and mannitol.
• Molecular Mass: The secreted recombinant mouse ACO2/GST chimera consists of 990 amino acids and has a calculated molecular mass of 110 kDa. The recombinant protein migrates as an approximately 100 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Aco2 aconitase 2, mitochondrial [ Mus musculus ]
• Official Symbol: ACO2
• Synonyms: ACO2; aconitase 2, mitochondrial; aconitate hydratase, mitochondrial; citrate hydro-lyase; Aco3; Aco-2; D10Wsu183e
• Gene ID: 11429
• mRNA Refseq: NM_080633
• Protein Refseq: NP_542364
• Pathway: Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem; Citrate cycle, first carbon oxidation, oxaloacetate =>2-oxoglutarate, organism-specific biosystem; Citrate cycle, first carbon oxidation, oxaloacetate
• Function: 3 iron, 4 sulfur cluster binding; 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; aconitate hydratase activity; citrate hydro-lyase (cis-aconitate-forming) activity; iron ion binding; iron-sulfur cluster binding; isocitrate hydro-lyase (ci