Recombinant Human ZNRF3 Protein (56-219 aa), His-SUMO-tagged

• Cat.No.: ZNRF3-1910H
• Product Overview: Recombinant Human ZNRF3 Protein (56-219 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Partial.

Specification

• Description: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.
• Source: E. coli
• Species: Human
• Tag: His&SUMO
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 34.2 kDa
• Protein length: 56-219 aa
• AA Sequence: KETAFVEVVLFESSPSGDYTTYTTGLTGRFSRAGATLSAEGEIVQMHPLGLCNNNDEEDLYEYGWVGVVKLEQPELDPKPCLTVLGKAKRAVQRGATAVIFDVSENPEAIDQLNQGSEDPLKRPVVYVKGADAIKLMNIVNKQKVARARIQHRPPRQPTEYFDM
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: ZNRF3 zinc and ring finger 3 [ Homo sapiens ]
• Official Symbol: ZNRF3
• Synonyms: RNF203; BK747E2.3
• Gene ID: 84133
• mRNA Refseq: NM_032173.3
• Protein Refseq: NP_115549.2
• MIM: 612062
• UniProt ID: Q9ULT6