Active Recombinant Human ZNRF3 protein, His-tagged

• Cat.No.: ZNRF3-2111H
• Product Overview: Recombinant Human ZNRF3 protein(Lys 56 - Met 219), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Lys 56 - Met 219
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Loaded Human ZNRF3, His Tag on HISIK Biosensor, can bind Human R-Spondin 3 Protein, Fc Tag with an affinity constant of 16.05 nM as determined in BLI assay (ForteBio Octet Red96e). Loaded Human R-Spondin 3 Protein, Fc Tag on Protein A Biosensor, can bind Human ZNRF3, His Tag with an affinity constant of 10.9 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 19.7 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ZNRF3 zinc and ring finger 3 [ Homo sapiens ]
• Official Symbol: ZNRF3
• Synonyms: RNF203; BK747E2.3
• Gene ID: 84133
• mRNA Refseq: NM_032173.3
• Protein Refseq: NP_115549.2
• MIM: 612062
• UniProt ID: Q9ULT6