Recombinant Human YOD1, His-tagged

• Cat.No.: YOD1-7575H
• Product Overview: Recombinant human YOD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-348aa
• Antigen Description: YOD1 is a highly conserved deubiquitinating enzyme of the ovarian tumor (otubain) family, whose function has yet to be assigned in mammalian cells. YOD1 is a constituent of a multiprotein complex with p97 as its nucleus, suggesting a functional link to a pathway responsible for the dislocation of misfolded proteins from the endoplasmic reticulum. Expression of a YOD1 variant deprived of its deubiquitinating activity imposes a halt on the dislocation reaction, as judged by the stabilization of various dislocation substrates.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
• Molecular Mass: 40.7 kDa (371aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMFGPAKG RHFGVHPAPG FPGGVSQQAA GTKAGPAGAW PVGSRTDTMW RLRCKAKDGT HVLQGLSSRT RVRELQGQIA AITGIAPGGQ RILVGYPPEC LDLSNGDTIL EDLPIQSGDM LIIEEDQTRP RSSPAFTKRG ASSYVRETLP VLTRTVVPAD NSCLFTSVYY VVEGGVLNPA CAPEMRRLIA QIVASDPDFY SEAILGKTNQ EYCDWIKRDD TWGGAIEISI LSKFYQCEIC VVDTQTVRID RFGEDAGYTK RVLLIYDGIH YDPLQRNFPD PDTPPLTIFS SNDDIVLVQA LELADEARRR RQFTDVNRFT LRCMVCQKGL TGQAEAREHA KETGHTNFGE V
• Purity: >90% as determined by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml

Gene Information

• Gene Name: YOD1?YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) [?Homo sapiens?]
• Official Symbol: YOD1
• Synonyms: YOD1; DUBA8; OTUD2; PRO0907; RP11-164O23.1; ubiquitin thioesterase OTU1; HIN-7; DUBA-8; hsHIN7; OTU domain containing 2; OTU domain containing 2; OTU domain-containing protein 2; YOD1 OTU deubiquinating enzyme 1 homolog ( yeast); EC 3.1.2; YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae); EC 3.4.19.12
• Gene ID: 55432
• mRNA Refseq: NM_018566
• Protein Refseq: NP_061036
• MIM: 612023
• UniProt ID: Q5VVQ6
• Chromosome Location: 1q32.2
• Pathway: Protein processing in endoplasmic reticulum
• Function: protein binding; ubiquitin-specific protease activity; zinc ion binding