Recombinant Human YOD1 Protein (F2-V348), His tagged

• Cat.No.: YOD1-0542H
• Product Overview: Recombinant Human 6His-TEV-GG-OTUD2(F2-V348 end) Protein was expressed in E. coli.

Specification

• Description: Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin. May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes. May recruit PLAA, UBXN6 and VCP to damaged lysosome membranes decorated with K48-linked ubiquitin chains and remove these chains allowing autophagosome formation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Protein length: F2-V348
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 80%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile. 20mM Tris-HCl (pH 8.0), 150mM NaCl, 10% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: YOD1 YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) [ Homo sapiens (human) ]
• Official Symbol: YOD1
• Synonyms: DUBA8; OTUD2; PRO0907; RP11-164O23.1
• Gene ID: 55432
• mRNA Refseq: NM_018566
• Protein Refseq: NP_061036
• MIM: 612023
• UniProt ID: Q5VVQ6