Recombinant Human XAF1, His-tagged

• Cat.No.: XAF1-185H
• Product Overview: XAF1, 1- 125aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1- 125aa
• Description: XAF1 is a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human XAF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 0.15M NaCl, 1mM DTT
• Molecular Mass: .6 kDa (161aa) confirmed by MALDI-TOF
• AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMEGD FSVCRNCKRH VVSANFTLHE AYCLRFLVLC PECEEPVPKE TMEEHCKLEH QQVGCTMCQQ SMQKSSLEFH KANECQERPV ECKFCKLDMQ LSKLELHESY CGSRTELCQG CGQFIMHRML A
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: XAF1 XIAP associated factor 1 [ Homo sapiens ]
• Official Symbol: XAF1
• Synonyms: XAF1; XIAP associated factor 1; XIAP-associated factor 1; BIRC4BP; HSXIAPAF1; XIAPAF1; BIRC4 binding protein; BIRC4-binding protein
• Gene ID: 54739
• mRNA Refseq: NM_017523
• Protein Refseq: NP_059993
• MIM: 606717
• UniProt ID: Q6GPH4
• Chromosome Location: 17p13.2
• Pathway: Cytokine Signaling in Immune system, organism-specific biosystem; Immune System, organism-specific biosystem; Interferon Signaling, organism-specific biosystem; Interferon alpha/beta signaling, organism-specific biosystem
• Function: metal ion binding; zinc ion binding