Recombinant Human WWTR1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: WWTR1-2240H
• Product Overview: WWTR1 MS Standard C13 and N15-labeled recombinant protein (NP_056287) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 44.1 kDa
• AA Sequence: MNPASAPPPLPPPGQQVIHVTQDLDTDLEALFNSVMNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKAMNQPLNHMNLHPAVSSTPVPQRSMAVSQPNLVMNHQHQQQMAPSTLSQQNHPTQNPPAGLMSMPNALTTQQQQQQKLRLQRIQMERERIRMRQEELMRQEAALCRQLPMEAETLAPVQAAVNPPTMTPDMRSITNNSSDPFLNGGPYHSREQSTDSGLGLGCYSVPTTPEDFLSNVDEMDTGENAGQTPMNINPQQTRFPDFLDCLPGTNVDLGTLESEDLIPLFNDVESALNKSEPFLTWLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: WWTR1 WW domain containing transcription regulator 1 [ Homo sapiens (human) ]
• Official Symbol: WWTR1
• Synonyms: WWTR1; WW domain containing transcription regulator 1; WW domain-containing transcription regulator protein 1; DKFZp586I1419; TAZ; transcriptional coactivator with PDZ-binding motif; transcriptional co-activator with PDZ-binding motif; FLJ27004; FLJ45718
• Gene ID: 25937
• mRNA Refseq: NM_015472
• Protein Refseq: NP_056287
• MIM: 607392
• UniProt ID: Q9GZV5