Recombinant human WWTR1, His-tagged

• Cat.No.: WWTR1-2480H
• Product Overview: Recombinant human WWTR1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Description: WWTR1 is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol
• Molecular Mass: 46.5kDa (423aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNPASAP PPLPPPGQQV IHVTQDLDTD LEALFNSVMN PKPSSWRKKI LPESFFKEPD SGSHSRQSSTDSSGGHPGPR LAGGAQHVRS HSSPASLQLG TGAGAAGSPA QQHAHLRQQS YDVTDELPLP PGWEMTFTAT GQRYFLNHIE KITTWQDPRKAMNQPLNHMN LHPAVSSTPV PQRSMAVSQP NLVMNHQHQQ QMAPSTLSQQ NHPTQNPPAG LMSMPNALTT QQQQQQKLRL QRIQMERERIRMRQEELMRQ EAALCRQLPM EAETLAPVQA AVNPPTMTPD MRSITNNSSD PFLNGGPYHS REQSTDSGLG LGCYSVPTTP EDFLSNVDEM DTGENAGQTP MNINPQQTRF PDFLDCLPGT NVDLGTLESE DLIPLFNDVE SALNKSEPFL TWL
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: WWTR1 WW domain containing transcription regulator 1 [ Homo sapiens ]
• Official Symbol: WWTR1
• Synonyms: WWTR1; WW domain containing transcription regulator 1; WW domain-containing transcription regulator protein 1; DKFZp586I1419; TAZ; transcriptional coactivator with PDZ-binding motif; transcriptional co-activator with PDZ-binding motif; FLJ27004; FLJ45718
• Gene ID: 25937
• mRNA Refseq: NM_001168278
• Protein Refseq: NP_001161750
• MIM: 607392
• UniProt ID: Q9GZV5
• Chromosome Location: 3q23-q24
• Pathway: Adipogenesis, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPARA Activates Gene Expression, organism-specific biosystem
• Function: protein binding; protein homodimerization activity; transcription coactivator activity; transcription coactivator activity; transcription corepressor activity