Recombinant Human Von Willebrand Factor

• Cat.No.: VWF-126H
• Product Overview: Recombinant Humanvon Willebrand factor protein expressed inE.coliencodes amino acids 2597-2791 and has a molecular mass of 48 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 2597-2791 a.a.
• Description: Factor VIII related antigen or von Willebrand factor is a multimeric glycoprotein. It has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. von Willebrand factor is synthesized by endothelial cells and stored in the Weibel-Palade granules. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. von Willebrand factor is one of the most useful markers to identify endothelial (or megakaryocytic) lineage of neoplasms. As not all endothelial cells synthesize / store) this molecule, about 30% of tumors of vascular origin fail to stain for factor VIII related antigen, regardless of whether they are benign or malignant. Staining for factor VIII related antigen has also been used to measure angiogenesis, an indicator of tumor recurrence.
• Formulation: Recombinant Human Factor VIII Related Antigen protein at 2µg/20ul 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
• Application: •ELISA •Inhibition Assays •Western Blotting.
• Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 48 kDa.
• Storage & Stability: Store vial at -20℃ to -80℃. When stored at the recommended temperature, this protein is stable for 12 months.

Gene Information

• Gene Name: VWF von Willebrand factor [ Homo sapiens ]
• Synonyms: VWF; von Willebrand factor; von Willebrand antigen 2; von Willebrand antigen II; F8VWF; F8; VWD; vWF; coagulation factor VIII VWF
• Gene ID: 7450
• mRNA Refseq: NM_000552
• Protein Refseq: NP_000543
• MIM: 193400
• UniProt ID: P04275
• Chromosome Location: 12p13.3
• Pathway: Complement and coagulation cascades; ECM-receptor interaction; Focal adhesion; Hemostasis; Integrin cell surface interactions
• Function: chaperone binding; collagen binding; glycoprotein binding; immunoglobulin binding; integrin binding; protease binding; protein N-terminus binding; protein homodimerization activity