Recombinant Human VHL, His-tagged

• Cat.No.: VHL-29832TH
• Product Overview: Recombinant fragment, corresponding to amino acids 1-154 of Human Von Hippel Lindau protein. This protein includes a His-tag at the N-terminal end.

Specification

• Description: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Conjugation: HIS
• Source: E. coli
• Tissue specificity: Expressed in the adult and fetal brain and kidney.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: PBS, 2mM EDTA, 1mM DTT, pH 7.4
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MPRRAENWDE AEVGAEEAGV EEYGPEEDGG EESGAEESGPEESGPEELGA EEEMEAGRPR PVLRSVNSRE PSQVIFCNRS PRVVLPVWLN FDGEPQPYPTLPPGTGRRIH SYRGHLWLFR DAGTHDGLLV NQTELFVPSL NVDGQPIFAN ITLP
• Protein length: 1-154 a.a.

Gene Information

• Gene Name: VHL von Hippel-Lindau tumor suppressor [ Homo sapiens ]
• Official Symbol: VHL
• Synonyms: VHL; von Hippel-Lindau tumor suppressor; von Hippel Lindau syndrome; von Hippel-Lindau disease tumor suppressor; VHL1
• Gene ID: 7428
• mRNA Refseq: NM_000551
• Protein Refseq: NP_000542
• MIM: 608537
• Uniprot ID: P40337
• Chromosome Location: 3p25.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem
• Function: protein binding; transcription factor binding