Recombinant Human VEGF 165 Protein, Ala27-Arg191

• Cat.No.: VEGF165-13H
• Product Overview: Recombinant Human VEGF 165 Protein (Ala27-Arg191) without tag was expressed in Spodoptera frugiperda, Sf 21 (baculovirus). Disulfide-linked homodimer.

Specification

• Species: Human
• Source: Insect cells
• Protein Length: Ala27-Arg191
• Bio-activity: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1-6 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 19.2 kDa (monomer)
  SDS-PAGE: 20-22 kDa, under reducing conditions. 39-42 kDa, under non-reducing conditions.
• Endotoxin: <0.01 EU/μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• N-terminal Sequence Analysis: Ala27
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. Alternatively, reconstitute at 100-500 μg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin.
• References: 1. Leung, D.W. et al. (1989) Science 246:1306.
  2. Keck, P.J. et al. (1989) Science 246:1309.
  3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens (human) ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; VPF; VEGF; MVCD1; L-VEGF; vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular permeability factor
• Gene ID: 7422
• mRNA Refseq: NM_001171626
• Protein Refseq: NP_001165097
• MIM: 192240
• UniProt ID: A0A0Y0IMM4