| Species : |
Human |
| Source : |
Sf9 Cells |
| Tag : |
GST |
| Description : |
LYN is a 56 kd tyrosine kinase that is similar to mouse T-lymphocyte-specific tyrosine kinase p56lck andthe v-yes protein as well as to the gene products of v-fgr and v-src. Northern hybridization analysisshowed that a 3.2-kilobase LYN mRNA was expressed in a variety of tissues of the human fetus. LYNis expressed preferentially in B cells and can be coimmunoprecipitated with IgM suggesting that LYN isphysically associated with membrane-bound IgM, and participates in antigen-mediated signaltransduction. Crosslinking of membrane-bound IgM with antibody induces rapid increase in activitiesof LYN and Lyn-associated phosphatidylinositol 3-kinase. Crosslinking of B-cell antigen receptor alsoinduces association of LYN with an 85-kDa noncatalytic subunit of phosphatidylinositol 3-kinase. Thus,LYN is functionally associated with membrane-bound IgM and participates in B-cell antigen receptormediatedsignaling. |
| Biological Activity : |
180 nmol phosphate incorporated into Poly(Glu-Tyr) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay). |
| Molecular Weight : |
85.0 kDa |
| Form : |
Recombinant protein in storage buffer (50 mMTris-HCl, pH 7.5, 150 mMNaCl, 0.25 mM DTT, 0.1 mMEGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol). |
| Appearance : |
Liquid |
| Purity : |
>98% by SDS-PAGE and HPLC analyses |
| Storage : |
Store product frozen at or below -70ºC. Stable for 1 year at -70ºC as undiluted stock. Aliquot to avoidrepeated thawing and freezing. |
| Pathways : |
B cell receptor signaling pathway; Chemokine signaling pathway; Epithelial cell signaling in Helicobacter pylori infection; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Hemostasis; Signaling in Immune system; Fc epsilon RI signaling pathway; IL-2 Signaling Pathway |
