Recombinant Human V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog, GST-tagged

• Cat.No.: LYN-1127H
• Product Overview: Recombinant human LYN was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Cat. No.: LYN-1127H
• Description: LYN is a 56 kd tyrosine kinase that is similar to mouse T-lymphocyte-specific tyrosine kinase p56lck andthe v-yes protein as well as to the gene products of v-fgr and v-src. Northern hybridization analysisshowed that a 3.2-kilobase LYN mRNA was expressed in a variety of tissues of the human fetus. LYNis expressed preferentially in B cells and can be coimmunoprecipitated with IgM suggesting that LYN isphysically associated with membrane-bound IgM, and participates in antigen-mediated signaltransduction. Crosslinking of membrane-bound IgM with antibody induces rapid increase in activitiesof LYN and Lyn-associated phosphatidylinositol 3-kinase. Crosslinking of B-cell antigen receptor alsoinduces association of LYN with an 85-kDa noncatalytic subunit of phosphatidylinositol 3-kinase. Thus,LYN is functionally associated with membrane-bound IgM and participates in B-cell antigen receptormediatedsignaling.
• Biological Activity: 180 nmol phosphate incorporated into Poly(Glu-Tyr) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
• Molecular Weight: 85.0 kDa
• Source: Sf9 insect cells
• Species: Human
• Form: Recombinant protein in storage buffer (50 mMTris-HCl, pH 7.5, 150 mMNaCl, 0.25 mM DTT, 0.1 mMEGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
• Appearance: Liquid
• Purity: >98% by SDS-PAGE and HPLC analyses
• Storage: Store product frozen at or below -70ºC. Stable for 1 year at -70ºC as undiluted stock. Aliquot to avoidrepeated thawing and freezing.
• Pathways: B cell receptor signaling pathway; Chemokine signaling pathway; Epithelial cell signaling in Helicobacter pylori infection; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Hemostasis; Signaling in Immune system; Fc epsilon RI signaling pathway; IL-2 Signaling Pathway

Gene Information

• Gene Name: LYN v-yes-1 Yamaguchi sarcoma viral related oncogene homolog [ Homo sapiens ]
• Official Symbol: LYN
• Synonyms: LYN; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog; JTK8; FLJ26625; Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog; EC 2.7.10.2; OTTHUMP00000226393; OTTHUMP00000226394
• Gene ID: 4067
• mRNA Refseq: NM_001111097
• Protein Refseq: NP_001104567
• MIM: 165120
• UniProt ID: P07948
• Chromosome Location: 8q13
• Function: ATP binding; glycosphingolipid binding; integrin binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; platelet-derived growth factor receptor binding; receptor signaling protein tyrosine kinase activity; transferase activity; ubiquitin protein ligase binding