Recombinant Human USP10 Protein (M1-E480), His/Strep tagged

• Cat.No.: USP10-0547H
• Product Overview: Recombinant Human GST-TEV-GG-USP10(A2-L798 end) Protein was expressed in E. coli.

Specification

• Description: Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Deubiquitinates TBX21 leading to its stabilization.
• Source: E. coli
• Species: Human
• Tag: His&Strep
• Form: Liquid
• Protein length: M1-E480
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: USP10 ubiquitin specific peptidase 10 [ Homo sapiens (human) ]
• Official Symbol: USP10
• Synonyms: USP10; ubiquitin specific peptidase 10; ubiquitin specific protease 10; ubiquitin carboxyl-terminal hydrolase 10; KIAA0190; UBPO; ubiquitin thioesterase 10; deubiquitinating enzyme 10; ubiquitin thiolesterase 10; ubiquitin-specific-processing protease 10; MGC2621
• Gene ID: 9100
• mRNA Refseq: NM_005153
• Protein Refseq: NP_005144
• MIM: 609818
• UniProt ID: Q14694