Active Recombinant Full Length Human USP10 Protein, C-6×His tagged

• Cat.No.: USP10-11HFL
• Product Overview: Recombinant Full Length Human USP10 Protein with a C-terminal 6×His tag was expressed in Spodoptera frugiperda, Sf 21 (Insect cells).

Specification

• Description: Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene.
• Source: Insect cells
• Species: Human
• Tag: His
• Bio-activity: Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-USP10 concentration of 20-100 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates.
• Molecular Mass: Predicted Molecular Mass: 89 kDa
• Protein length: 1-798 a.a.
• Purity: > 90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a solution in HEPES, NaCl, TCEP, and Glycerol.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: USP10 ubiquitin specific peptidase 10 [ Homo sapiens (human) ]
• Official Symbol: USP10
• Synonyms: USP10; ubiquitin specific peptidase 10; UBPO; ubiquitin carboxyl-terminal hydrolase 10; deubiquitinating enzyme 10; ubiquitin specific protease 10; ubiquitin thioesterase 10; ubiquitin thiolesterase 10; ubiquitin-specific-processing protease 10; EC 3.4.19.12
• Gene ID: 9100
• mRNA Refseq: NM_005153
• Protein Refseq: NP_005144
• MIM: 609818
• UniProt ID: Q14694