Recombinant Human UGT1A3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: UGT1A3-3375H
• Product Overview: UGT1A3 MS Standard C13 and N15-labeled recombinant protein (NP_061966) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. Substrates of this enzyme include estrone, 2-hydroxyestrone, and metabolites of benzo alpha-pyrene.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 57.3 kDa
• AA Sequence: MATGLQVPLPWLATGLLLLLSVQPWAESGKVLVVPIDGSHWLSMREVLRELHARGHQAVVLTPEVNMHIKEENFFTLTTYAISWTQDEFDRHVLGHTQLYFETEHFLKKFFRSMAMLNNMSLVYHRSCVELLHNEALIRHLNATSFDVVLTDPVNLCAAVLAKYLSIPTVFFLRNIPCDLDFKGTQCPNPSSYIPRLLTTNSDHMTFMQRVKNMLYPLALSYICHAFSAPYASLASELFQREVSVVDILSHASVWLFRGDFVMDYPRPIMPNMVFIGGINCANRKPLSQEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: UGT1A3 UDP glucuronosyltransferase family 1 member A3 [ Homo sapiens (human) ]
• Official Symbol: UGT1A3
• Synonyms: UGT1A3; UDP glucuronosyltransferase 1 family, polypeptide A3; UDP glycosyltransferase 1 family, polypeptide A3; UDP-glucuronosyltransferase 1-3; UGT1C; UGT1*3; UDP glucuronosyltransferase 1A3; UDP-glucuronosyltransferase 1-C; UDP-glucuronosyltransferase 1A3; UDPGT; UGT-1C; UGT1.3; UGT1-03; UDPGT 1-3
• Gene ID: 54659
• mRNA Refseq: NM_019093
• Protein Refseq: NP_061966
• MIM: 606428
• UniProt ID: P35503