Recombinant Human Ubiquitin-Conjugating Enzyme E2F (putative), His-tagged

• Cat.No.: UBE2F-2484H
• Product Overview: Recombinant human UBE2F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-185aa
• Description: UBE2F belongs to the ubiquitin-conjugating enzyme family and UBE2F subfamily. Ubiquitin-conjugating enzymes more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins.
• Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 30% glycerol, 1mM DTT, 200mM NaCl.
• Molecular Mass: 23.7kDa (209aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMLTLAS KLKRDDGLKG SRTAATASDS TRRVSVRDKL LVKEVAELEA NLPCTCKVHF PDPNKLHCFQ LTVTPDEGYY QGGKFQFETE VPDAYNMVPP KVKCLTKIWH PNITETGEIC LSLLREHSID GTGWAPTRTL KDVVWGLNSL FTDLLNFDDP LNIEAAEHHL RDKEDFRNKV DDYIKRYAR
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -78°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: UBE2F ubiquitin-conjugating enzyme E2F (UBC6 homolog, C. elegans) [ Homo sapiens ]
• Official Symbol: UBE2F
• Synonyms: UBE2F; ubiquitin-conjugating enzyme E2F (UBC6 homolog, C. elegans); NCE2; NEDD8 conjugating enzyme
• Gene ID: 23665
• UniProt ID: Q969M7
• Chromosome Location: 2q37.3
• Pathway: Cell cycle, organism-specific biosystem