Recombinant Human UBE2T, His-tagged

• Cat.No.: UBE2T-2355H
• Product Overview: Recombinant human UBE2T protein, fused to His-tag at C-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. 23.6 kDa (205aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: UBE2T, also known as PIG50 or HSPC150, is a member of the E2 ubiquitin-conjugating enzyme family Involved in the protein degradation pathway. This protein catalyzes the ATP-dependent attachment of ubiquitin (Ub) to target proteins, thereby tagging them for subsequent destruction by the proteasome. Additionally, UBE2T is thought to be a crucial component of the Faconi anemia pathway of DNA damage repair and, upon self-inactivation, may negatively regulate the Faconi pathway.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FK LE VIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDVLEH HHHHH.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UBE2T ubiquitin-conjugating enzyme E2T (putative) [ Homo sapiens ]
• Synonyms: ubiquitin-conjugating enzyme E2T (putative); PIG50; HSPC150; UBE2T; ubiquitin-conjugating enzyme E2 T; ubiquitin-protein ligase T; ubiquitin carrier protein T; ubiquitin conjugating enzyme; cell proliferation-inducing gene 50 protein; HSPC150 protein similar to ubiquitin-conjugating enzyme ; EC 6.3.2.19; Ubiquitin carrier protein T; Ubiquitin-protein ligase T
• Gene ID: 29089
• mRNA Refseq: NM_014176
• Protein Refseq: NP_054895
• MIM: 610538
• UniProt ID: Q9NPD8
• Chromosome Location: 1q32.1
• Pathway: DNA Repair
• Function: ubiquitin-protein ligase activity; ATP binding; ligase activity; nucleotide binding