Recombinant Human UBE2J1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: UBE2J1-4945H
• Product Overview: UBE2J1 MS Standard C13 and N15-labeled recombinant protein (NP_057105) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 35 kDa
• AA Sequence: METRYNLKSPAVKRLMKEAAELKDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSGSDSSQADQEAKELARQISFKAEVNSSGKTISESDLNHSFSLTDLQDDIPTTFQGATASTSYGVQNSSAASFHQPTQPVAKNTSMSPRQRRAQQQSQRRLSTSPDVIQGHQPRDNHTDHGGSAVLIVILTLALAALIFRRIYLANEYIFDFELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: UBE2J1 ubiquitin conjugating enzyme E2 J1 [ Homo sapiens (human) ]
• Official Symbol: UBE2J1
• Synonyms: UBE2J1; ubiquitin-conjugating enzyme E2, J1, U; ubiquitin conjugating enzyme E2, J1 (UBC6 homolog, yeast); ubiquitin-conjugating enzyme E2 J1; CGI 76; HSPC153; NCUBE1; UBC6; HSUBC6e; non-canonical ubiquitin-conjugating enzyme 1; yeast ubiquitin-conjugating enzyme UBC6 homolog E; ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast); Ubc6p; CGI-76; HSPC205; NCUBE-1; HSU93243; MGC12555
• Gene ID: 51465
• mRNA Refseq: NM_016021
• Protein Refseq: NP_057105
• MIM: 616175
• UniProt ID: Q9Y385