Recombinant Human UBE2I Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: UBE2I-4545H
• Product Overview: UBE2I MS Standard C13 and N15-labeled recombinant protein (NP_919237) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 18 kDa
• AA Sequence: MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: UBE2I ubiquitin-conjugating enzyme E2I [ Homo sapiens (human) ]
• Official Symbol: UBE2I
• Synonyms: UBE2I; ubiquitin-conjugating enzyme E2I; ubiquitin conjugating enzyme E2I (homologous to yeast UBC9), ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast); SUMO-conjugating enzyme UBC9; UBC9; SUMO-protein ligase; SUMO-1-protein ligase; ubiquitin-protein ligase I; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin-protein ligase E2I; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); P18; C358B7.1
• Gene ID: 7329
• mRNA Refseq: NM_194261
• Protein Refseq: NP_919237
• MIM: 601661
• UniProt ID: P63279