Recombinant Human UBE2E3, His-tagged

• Cat.No.: UBE2E3-112H
• Product Overview: Recombinant human UBE2E3, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: UBE2E3 also known as Ubiquitin-conjugating enzyme E2 E3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. The ubiquitination process covalently attaches ubiquitin, a short protein of 76 amino acids, to a lysine residue on the target protein. Once a protein has been tagged with one ubiquitin molecule, additional rounds of ubiquitination form a polyubiquitin chain that is recognized by the proteasome"s 19S regulatory particle, triggering the ATP-dependent unfolding of the target protein that allows passage into the proteasome"s 20S core particle, where proteases degrade the target into short peptide fragments for recycling by the cell.
• Form: Liquid, In Phosphate buffered saline (pH7.4) containing 20% glycerol, 1mM DTT
• Molecular Mass: 25.3kDa (230aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSDRQR SDDESPSTSS GSSDADQRDP AAPEPEEQEE RKPSATQQKK NTKLSSKTTA KLSTSAKRIQKELAEITLDP PPNCSAGPKG DNIYEWRSTI LGPPGSVYEG GVFFLDITFS SDYPFKPPKV TFRTRIYHCN INSQGVICLD ILKDNWSPAL TISKVLLSIC SLLTDCNPAD PLVGSIATQY LTNRAEHDRI ARQWTKRYAT
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: UBE2E3 ubiquitin-conjugating enzyme E2E 3 [ Homo sapiens ]
• Official Symbol: UBE2E3
• Synonyms: UBE2E3; ubiquitin-conjugating enzyme E2E 3; ubiquitin conjugating enzyme E2E 3 (homologous to yeast UBC4/5) , ubiquitin conjugating enzyme E2E 3 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2 E3; UbcH9; ubiquitin-protein ligase E3; ubiquitin carrier protein E3; ubiquitin-conjugating enzyme E2-23 kDa; ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5); UBCH9; UbcM2
• Gene ID: 10477
• mRNA Refseq: NM_006357
• Protein Refseq: NP_006348
• MIM: 604151
• UniProt ID: Q969T4
• Chromosome Location: 2q31.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem
• Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; ubiquitin-protein ligase activity