Recombinant Human UBA3 Protein, His-tagged

• Cat.No.: UBA3-522H
• Product Overview: Recombinant Human UBA3 Protein (Ser241-Lys458) with N-His tag was expressed in E. coli.

Specification

• Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.1 kDa
  Accurate Molecular Mass: 28 kDa
• Protein length: Ser241-Lys458
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: UBA3 ubiquitin-like modifier activating enzyme 3 [ Homo sapiens (human) ]
• Official Symbol: UBA3
• Synonyms: UBA3; ubiquitin-like modifier activating enzyme 3; UBE1C, ubiquitin activating enzyme E1C (homologous to yeast UBA3), ubiquitin activating enzyme E1C (UBA3 homolog, yeast); NEDD8-activating enzyme E1 catalytic subunit; hUba3; ubiquitin activating enzyme E1 homolog (yeast); NEDD8-activating enzyme E1C; Nedd8-activating enzyme hUba3; ubiquitin-activating enzyme 3; ubiquitin-like modifier-activating enzyme 3; UBA3, ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1C (UBA3 homolog, yeast); ubiquitin-activating enzyme E1C (homologous to yeast UBA3); UBE1C; hUBA3; MGC22384; DKFZp566J164
• Gene ID: 9039
• mRNA Refseq: NM_003968
• Protein Refseq: NP_003959
• MIM: 603172
• UniProt ID: Q8TBC4