Recombinant Human UBA1 protein(Ser2-Arg1058), His&GST-tagged

• Cat.No.: UBA1-7333H
• Product Overview: Recombinant Human UBA1 (NP_003325.2) (Ser 2-Arg 1058) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His&GST
• Protein length: Ser2-Arg1058
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4, 10% gly, 0.5mM GSH Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human UBA1/GST chimera consists of 1294 amino acids and has a calculated molecular mass of 146 KDa. It migrates as an approximately 130 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: UBA1 ubiquitin-like modifier activating enzyme 1 [ Homo sapiens ]
• Official Symbol: UBA1
• Synonyms: UBA1; ubiquitin-like modifier activating enzyme 1; A1S9T, GXP1, UBE1, ubiquitin activating enzyme E1 , ubiquitin activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing); ubiquitin-like modifier-activating enzyme 1; POC20; POC20 centriolar protein homolog (Chlamydomonas); ubiquitin activating enzyme E1 homolog (yeast); UBE1X; POC20 centriolar protein homolog; UBA1, ubiquitin-activating enzyme E1 homolog A; A1S9T and BN75 temperature sensitivity complementing; A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; SMAX2; UBA1A; MGC4781
• Gene ID: 7317
• mRNA Refseq: NM_003334
• Protein Refseq: NP_003325
• MIM: 314370
• UniProt ID: P22314