Recombinant Human Tyrosyl-tRNA Synthetase 2, Mitochondrial, His-tagged

• Cat.No.: YARS2-6783H
• Product Overview: Recombinant humanYARS2 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: YARS2, also known astyrosyl-tRNA synthetase, catalyzes the attachment of tyrosine to tRNA(Tyr) ina two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP andthen transferred to the acceptor end of tRNA(Tyr)
• Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
• Molecular Weight: 53.7 kDa (482aa)
• Purity: > 85% by SDS -PAGE
• Concentration: 0.25 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MTLNLSVLLP LGLRKAHSGA QGLLAAQKAR GLFKDFFPET GTKIELPELF DRGTASFPQTIYCGFDPTAD SLHVGHLLAL LGLFHLQRAG HNVIALVGGA TARLGDPSGR TKEREALETE RVRANARALRLGLEALAANH QQLFTDGRSW GSFTVLDNSA WYQKQHLVDF LAAVGGHFRM GTLLSRQSVQ LRLKSPEGMSLAEFFYQVLQ AYDFYYLFQR YGCRVQLGGS DQLGNIMSGY EFINKLTGED VFGITVPLIT STTGAKLGKSAGNAVWLNRD KTSPFELYQF FVRQPDDSVE RYLKLFTFLP LPEIDHIMQL HVKEPERRGP QKRLAAEVTKLVHGREGLDS AKRCTQALYH SSIDALEVMS DQELKELFKE APFSEFFLDP GTSVLDTCRK ANAIPDGPRGYRMITEGGVS INHQQVTNPE SVLIVGQHIL KNGLSLLKIG KRNFYIIKWL QL
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: YARS2tyrosyl-tRNA synthetase 2, mitochondrial [ Homo sapiens ]
• Official Symbol: YARS2
• Synonyms: YARS2; tyrosyl-tRNA synthetase 2,mitochondrial; tyrosyl-tRNA synthetase, mitochondrial; CGI 04; FLJ13995; mtTyrRS; tyrosine tRNA ligase 2; mitochondrial; CGI 04; FLJ13995; mt TyrRS;Tyrosine tRNA ligase; Tyrosyl tRNA synthetase 2, mitochondrial; TYRRS; YARS2; tyrosine tRNA ligase 2, mitochondrial; TYRRS; CGI-04; MLASA2; MT-TYRRS
• Gene ID: 51067
• mRNA Refseq: NM_001040436
• Protein Refseq: NP_001035526
• MIM: 610957
• Uniprot ID: Q9Y2Z4
• Chromosome Location: 12p11.21
• Pathway: Aminoacyl-tRNA biosynthesis,organism-specific biosystem; Aminoacyl-tRNA biosynthesis, conservedbiosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, organism-specificbiosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, conserved biosystem; GeneExpression, organism-specific biosystem
• Function: ATP binding; ligase activity;nucleotide binding; protein homodimerization activity; tRNA binding