Recombinant Human Tumor Susceptibility Gene 101, His-tagged

• Cat.No.: TSG101-2357H
• Product Overview: Recombinant human TSG101 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. 20.7 kDa (181aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: TSG101(Tumor susceptibility gene 101) protein belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. This protein contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. Mutations and alternative splicing in this protein occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol.
• Purity: > 90% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAVS ESQLKKMVSK YKYRDLTVRE TVNVITLYKD LKPVLDSYVF NDGSSRELMNLTGTIPVPYR GNTY NIPICL WLLDTYPYNP PICFVKPTSS MTIKTGKHVD ANGKIYLPYL HEWKHPQSDL LGLIQVMIVV FGDEPPVFSRP.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: TSG101 tumor susceptibility gene 101 [ Homo sapiens ]
• Synonyms: tumor susceptibility gene 101; TSG10; VPS23; TSG101; tumor susceptibility gene 101 protein; tumor susceptibility protein; ESCRT-I complex subunit TSG101; tumor susceptibility gene 10; tumor susceptibility protein
• Gene ID: 7251
• mRNA Refseq: NM_006292
• Protein Refseq: NP_006283
• MIM: 601387
• UniProt ID: Q99816
• Chromosome Location: 11p15
• Pathway: Endocytosis
• Function: ligand-dependent nuclear receptor transcription coactivator activity; DNA binding; calcium-dependent protein binding; protein binding; small conjugating protein ligase activity; transcription corepressor activity; ubiquitin binding