Recombinant Human Tropomyosin 3, His-tagged

• Cat.No.: TPM3-4853H
• Product Overview: Recombinant human TPM3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: TPM3-4853H
• Description: Tropomyosin alpha-3 chain, also known as TPM3, is member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing1mM DTT, 10% glycerol, 0.1M NaCl.
• Molecular Weight: 31.6 kDa (272aa), confirmed by MALDI-TOF.
• Purity: >90% by SDS-PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMAGITT IEAVKRKIQV LQQQADDAEE RAERLQREVE GERRAREQAE AEVASLNRRI QLVEEELDRA QERLATALQK LEEAEKAADE SERGMKVIEN RALKDEEKME LQEIQLKEAK HIAEEADRKY EEVARKLVII EGDLERTEER AELAESRCRE MDEQIRLMDQ NLKCLSAAEE KYSQKEDKYE EEIKILTDKL KEAETRAEFA ERSVAKLEKT IDDLEDKLKC TKEEHLCTQR MLDQTLLDLN EM
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Cardiac muscle contraction; Dilated cardiomyopathy; Hypertrophic cardiomyopathy (HCM); Muscle contraction; Pathways in cancer; Smooth Muscle Contraction; Striated Muscle Contraction; Thyroid cancer
• Protein length: 1-248 a.a.

Gene Information

• Gene Name: TPM3 tropomyosin 3 [ Homo sapiens ]
• Official Symbol: TPM3
• Synonyms: TPM3; tropomyosin 3; TM3; TRK; NEM1; TM-5; TM30; TM30nm; TPMsk3; hscp30; MGC3261; FLJ41118; MGC14582; MGC72094; OK/SW-cl.5; tropomyosin alpha-3 chain; hTM5; tropomyosin-3; tropomyosin-5; gamma-tropomyosin; tropomyosin gamma; OTTHUMP00000034016; OTTHUMP00000034018; OTTHUMP00000034019; OTTHUMP00000034020; OTTHUMP00000034021; OTTHUMP00000034171; OTTHUMP00000034172; OTTHUMP00000034173; OTTHUMP00000218067; OTTHUMP00000218071; cytoskeletal tropomyosin TM30; heat-stable cytoskeletal protein 30 kDa; Gamma-tropomyosin; Tropomyosin-3; Tropomyosin-5
• Gene ID: 7170
• mRNA Refseq: NM_001043351
• Protein Refseq: NP_001036816
• MIM: 191030
• UniProt ID: P06753
• Chromosome Location: 1q21.2
• Function: actin binding; molecular function