Recombinant Human triggering receptor expressed on myeloid cells 2 Protein, His&StrepII tagged

• Cat.No.: TREM2-088H
• Product Overview: Recombinant human TREM2 protein (Q9NZC2) (His 19-Ser 174) with a Strep II tag at the N-terminus and a His tag at the C-terminus was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: N-StrepII and C-His
• ProteinLength: 19-174aa
• Description: Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding. Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia. Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1. Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia. Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin. Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade. Required for microglial phagocytosis of apoptotic neurons. Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain. Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide. It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB. During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling. Plays a role in microglial MTOR activation and metabolism. Regulates age-related changes in microglial numbers.
• Tag: N-StrepII and C-His
• Molecular Mass: 20.2 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: TREM2 triggering receptor expressed on myeloid cells 2 [ Homo sapiens (human) ]
• Official Symbol: TREM2
• Synonyms: TREM2; triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2a; TREM 2; Trem2a; Trem2b; Trem2c; triggering receptor expressed on monocytes 2; TREM-2
• Gene ID: 54209
• mRNA Refseq: NM_018965
• Protein Refseq: NP_061838
• MIM: 605086
• UniProt ID: Q9NZC2