Recombinant Human Transcription Factor B2, Mitochondrial, His-tagged

• Cat.No.: TFB2M-7205H
• Product Overview: Recombinant human TFB2M protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 45.8 kDa.

Specification

• Description: TFB2M is S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. This protein is also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. TFB2M stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT.
• Molecular Mass: 45.8 kDa (401aa) confirmed by MALDI-TOF
• Protein length: 20-396aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGRFCI LGSEAATRKH LPARNHCGLS DSSPQLWPEP DFRNPPRKAS KASLDFKRYV TDRRLAETLA QIYLGKPSRP PHLLLECNPG PGILTQALLE AGAKVVALES DKTFIPHLES LGKNLDGKLR VIHCDFFKLD PRSGGVIKPP AMSSRGLFKN LGIEAVPWTA DIPLKVVGMF PSRGEKRALW KLAYDLYSCT SIYKFGRIEV NMFIGEKEFQ KLMADPGNPD LYHVLSVIWQ LACEIKVLHM EPWSSFDIYT RKGPLENPKR RELLDQLQQK LYLIQMIPRQ NLFTKNLTPM NYNIFFHLLK HCFGRRSATV IDHLRSLTPL DARDILMQIG KQEDEKVVNM HPQDFKTLFE TIERSKDCAY KWLYDETLED R
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TFB2M transcription factor B2, mitochondrial [ Homo sapiens ]
• Official Symbol: TFB2M
• Synonyms: TFB2M; transcription factor B2, mitochondrial; dimethyladenosine transferase 2, mitochondrial; FLJ22661; FLJ23182; Hkp1; hTFB2M; h-mtTFB; h-mtTFB2; HCV NS5A-transactivated protein 5; mitochondrial 12S rRNA dimethylase 2; mitochondrial transcription factor B2; hepatitis C virus NS5A-transactivated protein 5; S-adenosylmethionine-6-N, N-adenosyl(rRNA) dimethyltransferase 2; mtTFB2
• Gene ID: 64216
• mRNA Refseq: NM_022366
• Protein Refseq: NP_071761
• MIM: 607055
• UniProt ID: Q9H5Q4
• Chromosome Location: 1q44
• Pathway: Energy Metabolism, organism-specific biosystem; Gene Expression, organism-specific biosystem; Mitochondrial Gene Expression, organism-specific biosystem; Mitochondrial transcription initiation, organism-specific biosystem; RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription, organism-specific biosystem; Transcription from mitochondrial promoters, organism-specific biosystem
• Function: methyltransferase activity; rRNA (adenine-N6,N6-)-dimethyltransferase activity; rRNA methyltransferase activity; transcription cofactor activity; transferase activity